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Transcriptome of an Armillaria root disease pathogen reveals candidate genes involved in host substrate utilization at the host-pathogen interface

Posted date: April 22, 2014
Publication Year: 
2013
Authors: Ross-Davis, Amy; Stewart, J. E.; Hanna, John W.; Kim, M.-S.; Knaus, B. J.; Cronn, R.; Rai, H.; Richardson, Bryce A.; McDonald, G. I.; Klopfenstein, Ned B.
Publication Series: 
Scientific Journal (JRNL)
Source: Forest Pathology. 43: 468-477.

Abstract

Armillaria species display diverse ecological roles ranging from beneficial saprobe to virulent pathogen. Armillaria solidipes (formerly A. ostoyae), a causal agent of Armillaria root disease, is a virulent primary pathogen with a broad host range of woody plants across the Northern Hemisphere. This white-rot pathogen grows between trees as rhizomorphs and attacks sapwood as mycelial fans under the bark. Armillaria root disease is responsible for reduced forest productivity due to direct tree mortality and non-lethal infections that impact growth. Here, we characterize a transcriptome of a widespread, virulent genet (vegetative clone) of A. solidipes isolated from a mycelial fan on a natural grand fir (Abies grandis) sapling in northern Idaho, USA. cDNA from polyA+-purified total RNA was sequenced using a singleend read approach on the Illumina GAIIx platform which generated 24 170 384 reads. A BLASTx search against the NCBI nr database using 39 943 de novo assembled contigs resulted in 24 442 sequences with significant hits (e-value

Citation

Ross-Davis, A. L.; Stewart, J. E.; Hanna, J. W.; Kim, M.-S.; Knaus, B. J.; Cronn, R.; Rai, H.; Richardson, B. A.; McDonald, G. I.; Klopfenstein, N. B. 2013. Transcriptome of an Armillaria root disease pathogen reveals candidate genes involved in host substrate utilization at the host-pathogen interface. Forest Pathology. 43: 468-477.