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An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

Posted date: September 12, 2016
Publication Year: 
2016
Publication Series: 
Scientific Journal (JRNL)
Source: Conservation Genetics Resources. doi: 10.1007/s12686-016-0531-1.

Abstract

The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling (Thymallus arcticus) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DNA in environmental samples. The assay amplified low concentrations of Arctic grayling DNA consistently, and did not amplify non-target species, including sympatric salmonid fishes.

Citation

Carim, K. J.; Dysthe, J. C. S.; Young, M. K.; McKelvey, K. S.; Schwartz, M. K. 2016. An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America. Conservation Genetics Resources. doi: 10.1007/s12686-016-0531-1.