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Bioconversion of Beetle-Killed Lodgepole Pine Using SPORL: Process Scale-up Design, Lignin Coproduct, and High Solids Fermentation without detoxification

Posted date: December 17, 2013
Publication Year: 
2013
Authors: Zhou, Haifeng; Zhu, J.Y.; Luo, Xiaolin; Leu, Shao-Yuan; Wu, Xiaolei; Gleisner, Roland; Dien, Bruce S.; Hector, Ronald E.; Yang, Dongjie; Qiu, Xueqing; Horn, Eric; Negron, Jose
Publication Series: 
Scientific Journal (JRNL)
Source: Industrial and Engineering Chemistry Research. 52, pp. 16057-16065.

Abstract

Mountain pine beetle killed Lodgepole pine (Pinus contorta Douglas ex Loudon) wood chips were pretreated using an acidic sulfite solution of approximately pH = 2.0 at a liquor to wood ratio of 3 and sodium bisulfite loading of 8 wt % on wood. The combined hydrolysis factor (CHF), formulated from reaction kinetics, was used to design a scale-up pretreatment on 2000 g wood chips at a relatively low temperature of 165 °C that reduced furan formation and facilitated high solids saccharification and fermentation. The pretreated solids and liquor were disk milled together to result in a biomass whole slurry of 25% total solids. The whole biomass slurry was directly used to conduct simultaneous enzymatic saccharification and combined fermentation (SSCombF) using a commercial cellulase and Saccharomyces cerevisiae YRH400 without detoxification. A terminal ethanol titer of 47.1 g L−1 with a yield of 306 L (tonne wood)−1, or 72.0% theoretical, was achieved when SSCombF was conducted at an unwashed solids loading of 18%. The lignosulfonate (LS) from SPORL was highly sulfonated and showed better dispersibility than a high purity commercial softwood LS, and therefore has potential as a directly marketable coproduct.

Citation

Zhou, Haifeng; Zhu, J.Y.; Luo, Xiaolin; Leu, Shao-Yuan; Wu, Xiaolei; Gleisner, Roland; Dien, Bruce S.; Hector, Ronald E.; Yang, Dongjie; Qiu, Xueqing; Horn, Eric; Negron, Jose. 2013. Bioconversion of Beetle-Killed Lodgepole Pine Using SPORL: Process Scale-up Design, Lignin Coproduct, and High Solids Fermentation without detoxification. Industrial and Engineering Chemistry Research. 52, pp. 16057-16065.