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Joseph Dysthe


Publications

Franklin, Thomas; Wilcox, Taylor M.; McKelvey, Kevin S.; Greaves, Samuel; Dysthe, Joseph; Young, Michael K.; Schwartz, Michael K., 2019. Repurposing environmental DNA samples to verify the distribution of Rocky Mountain tailed frogs in the Warm Springs Creek Basin, Montana
Franklin, Thomas; McKelvey, Kevin S.; Golding, Jessie; Mason, Daniel H.; Dysthe, Joseph; Pilgrim, Kristine L.; Squires, John R.; Aubry, Keith B.; Long, Robert A.; Greaves, Samuel; Raley, Catherine M.; Jackson, Scott; MacKay, Paula; Lisbon, Joshua; Sauder, Joel D.; Pruss, Michael T.; Heffington, Don; Schwartz, Michael K., 2019. Using environmental DNA methods to improve winter surveys for rare carnivores: DNA from snow and improved noninvasive techniques
Carim, Kellie; Dysthe, Joseph; McLellan, Holly; Young, Michael K.; McKelvey, Kevin S.; Schwartz, Michael K., 2019. Using environmental DNA sampling to monitor the invasion of nonnative Esox lucius (northern pike) in the Columbia River basin, USA
Franklin, Thomas; Dysthe, Joseph; Rubenson, Erika S.; Carim, Kellie; Olden, Julian D.; McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K., 2018. A non-invasive sampling method for detecting non-native smallmouth bass (Micropterus dolomieu)
Dysthe, Joseph; Franklin, Thomas; McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K., 2018. An improved environmental DNA assay for bull trout (Salvelinus confluentus) based on the ribosomal internal transcribed spacer I
Franklin, Thomas; Dysthe, Joseph; Golden, Michael; McKelvey, Kevin S.; Hossack, Blake R.; Carim, Kellie; Tait, Cynthia; Young, Michael K.; Schwartz, Michael K., 2018. Inferring presence of the western toad (Anaxyrus boreas) species complex using environmental DNA
Dysthe, Joseph; Carim, Kellie; Franklin, Thomas; Kikkert, Dave; Young, Michael K.; McKelvey, Kevin S.; Schwartz, Michael K., 2018. Molecular detection of northern leatherside chub (Lepidomeda copei) DNA in environmental samples
Dysthe, Joseph; Rodgers, Torrey; Franklin, Thomas; Carim, Kellie; Young, Michael K.; McKelvey, Kevin S.; Mock, Karen E.; Schwartz, Michael K., 2018. Repurposing environmental DNA samples: Detecting the western pearlshell (Margaritifera falcata) as a proof of concept
Mason, Daniel H.; Dysthe, Joseph; Franklin, Thomas; Skorupski, Joseph A.; Young, Michael K.; McKelvey, Kevin S.; Schwartz, Michael K., 2018. qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples
Carim, Kellie; Dysthe, Joseph; Young, Michael K.; McKelvey, Kevin S.; Schwartz, Michael K., 2017. A noninvasive tool to assess the distribution of Pacific lamprey (Entosphenus tridentatus) in the Columbia River basin
Dysthe, Joseph; Carim, Kellie; Ruggles, Michael; McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K., 2017. Environmental DNA assays for the sister taxa aauger (Sander canadensis) and walleye (Sander vitreus)
Carim, Kellie; Dysthe, Joseph; Young, Michael K.; McKelvey, Kevin S.; Schwartz, Michael K., 2016. An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America
Dysthe, Joseph; Carim, Kellie; Paroz, Yvette M.; McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K., 2016. Quantitative PCR assays for detecting loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) in the southwestern United States
Effective conservation and management decisions for habitats require information about the distribution of multiple species but such data is expensive to obtain; this often limits data collection to just a few, high-profile species. Environmental DNA (eDNA) sampling can be more sensitive, and less expensive, than traditional sampling for aquatic species, and a single sample potentially contains DNA from all species present in a waterbody. Cost-savings accrue if eDNA collected for detecting a particular species can be repurposed to detect additional species. This study tested the feasibility of repurposing and re-analyzing already collected samples.   
The website provides: 1) A large list of supporting science behind eDNA sampling. 2) The recommended field protocol for eDNA sampling and the equipment loan program administered by the NGC. 3) A systematically-spaced sampling grid for all flowing waters of the U.S. in a downloadable format that includes unique database identifiers and geographic coordinates for all sampling sites. Available for download in an Geodatabase or available by ArcGIS Online map. This sampling grid can be used to determine your field collection sites to contribute. 4) The lab results of eDNA sampling at those sites where project partners have agreed to share data.

RMRS Science Program Areas: 
Wildlife and Terrestrial Ecosystems