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Keyword: DNA

qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples

Publications Posted on: April 05, 2019
The Sturgeon chub (Macrhybopsis gelida) is a cyprinid fish native to the Missouri and Mississippi River basins of the U.S. Suspected long-term declines in the size of its distribution have prompted a review of its conservation status by the U.S. Fish and Wildlife Service, a process which depends on reliable methods to delineate the distribution and status of extant populations.

A new way to look at winter footprints

FS News Posted on: November 26, 2018
An innovative new project has discovered that animal footprints contain enough DNA to allow for species identification. Scientists have traditionally relied on snow-tracks and camera traps to monitor populations of rare carnivores, like Canada lynx, fishers and wolverines. These traditional techniques can tell part of, but not the entire story of an animal population, and are sometimes difficult to validate species identification. 

Toward a west-wide model of Armillaria root disease: New surveys needed in western Oregon, western Washington, and Alaska

Publications Posted on: December 12, 2017
Currently, Armillaria root disease causes large growth/volume losses (e.g., 16-55%) in areas of western North America (Filip and Goheen 1984; Cruickshank 2011; Lockman and Kearns 2016). Armillaria root disease is typically more severe in trees that are maladapted to climate-induced stress (Ayres and Lombardero 2000; Kliejunas et al. 2009; Sturrock 2011).

Scat-detection dogs survey low density moose in New York

Publications Posted on: November 18, 2016
The difficulty of collecting occurrence and population dynamics data in mammalian populations of low density poses challenges for making informed management decisions. We assessed the use of scat-detection dogs to search for fecal pellets in a low density moose (Alces alces) population in the Adirondack Park in New York State, and the success rate of DNA extraction from moose fecal pellets collected during the surveys.

Quantitative PCR assays for detecting loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) in the southwestern United States

Publications Posted on: September 12, 2016
Loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) are legally protected with the status of Endangered under the U.S. Endangered Species Act and are endemic to the Gila River basin of Arizona and New Mexico. Efficient and sensitive methods for monitoring these species’ distributions are critical for prioritizing conservation efforts.

Headwater streams are resistant to trout hybridization

Science Spotlights Posted on: August 24, 2016
Hybridization between westslope cutthroat trout and both rainbow trout and Yellowstone cutthroat trout is a major conservation concern for the species.  A new broad-scale analysis of hybridization patterns found many pure populations of westslope cutthroat trout in headwaters streams.

Fisher survey protocol

Documents and Media Posted on: May 23, 2016
Rocky Mountain Research Station scientists and their partners established survey protocol for studying the geographic range of fisher in the Rocky Mountains and studying their DNA. Document Type: White Papers

Using a metagenomic approach to improve our understanding of Armillaria root disease

Publications Posted on: March 26, 2016
Metagenomics has illuminated our understanding of how microbial communities influence health and disease. Researchers are beginning to characterize what constitutes healthy microbiota in terms of structure, function, and diversity in a variety of environments.

Bioclimatic models estimate areas with suitable climate for Armillaria spp. in Wyoming

Publications Posted on: March 26, 2016
Armillaria species range from beneficial saprobes to damaging root pathogens, and their ecological roles and impacts vary with environment and host. Armillaria solidipes [pending vote to conserve A. ostoyae . (Redhead et al. 2011 )] is known as an aggressive pathogen of conifers and causes tree mortality and significant growth loss in Wyoming and throughout the world.

Preliminary survey of wood-associated fungi in southeast O'ahu of Hawai'i using DNA-based identification

Publications Posted on: March 26, 2016
Hawai'i is a biological hotspot with a variety of climates and habitats. While fungal species diversity has been more extensively studied in Hawai'i than other Pacific Islands (e.g. see Gilbertson et al. 2002), there remain many species unreported in the literature. This project attempted to capture a small portion of Hawai'i's fungal diversity in southeast O'ahu by identifying fruit bodies using molecular techniques.

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