M.E. Casey Crooks
One Gifford Pinchot Drive
Contact M.E. Casey Crooks
Our research goals are to use genetic tools to identify important genes and gene products involved in wood decay and fermentation of cellulosic biomass. We seek to use a large scale genetic screening approach dependent on the development of high throughput transformation and subsequent high throughput phenotypic assays. This mass screening approach seeks to compliment the currently deployed biochip array and genomics based informatics to identify potentially valuable candidate genes and enzymes that might not otherwise be recognized.
- Molecular cloning and characterization of pyranose 2-oxidase gene from the brown rot fungus Gloeophyllum trabeum
- Molecular genetics of filamentous fungi, particularly Basidiomycetes
- Forward genetic identification of elements influencing ethanol production in Pichia stipitis for use in strain improvement.
- Functional genomics and bioinformatics related to lignocellulose-degrading fungi
- Improved transformation technologies for manipulation of white and brown rot fungi using biolistic bombardment.
- Whole genome expression microarray analysis and proteomics
- Heterologous expression systems
- Phanerochaete chrysosporium, Postia placenta, Ceriporiopsis subvermispora
Featured Publications & Products
- Dietrich, Diane; Crooks, Casey. 2009. Gene cloning and heterologous expression of pyranose 2-oxidase from the brown-rot fungus, Gloeophyllum trabeum.
- Fan, Jun; Hill, Lionel; Crooks, Casey; Doerner, Peter; Lamb, Chris. 2009. Abscisic acid has a key role in modulating diverse plant-pathogen interactions.
- St John, Franz; Dietrich, Diane; Crooks, Casey. . Glycosyl Hydrolase Xylanases, Compositions and Methods of Use for Efficient Hydrolysis and Processing of Xylan.
- St. John, Franz; Dietrich, Diane; Crooks, Casey. 2018. Glycosyl Hydrolase Xylanases, Compositions and Methods of Use for Efficient Hydrolysis and Processing of Xylan.
- Crooks, Casey ; Long, Liangkun ; St John, Franz J. 2020. CaXyn30B from the solventogenic bacterium Clostridium acetobutylicum is a glucuronic acid-dependent endoxylanase.
- St John, Franz J.; Dietrich, Diane ; Crooks, Casey ; Balogun, Peter ; de Serrano, Vesna ; Pozharski, Edwin ; Smith, James Kennon; Bales, Elizabeth ; Hurlbert, Jason . 2018. A plasmid borne, functionally novel glycoside hydrolase family 30 subfamily 8 endoxylanase from solventogenic Clostridium.
- Crooks, Casey ; Palmer, Jonathan M.; Lindner, Daniel L. 2018. Draft genome sequence of Burkholderia cepacia ATCC 17759, a polyhydroxybutyrate-co-valerate copolymer-producing bacterium.
- St John, Franz J.; Crooks, Casey; Dietrich, Diane; Hurlbert, Jason. 2017. Xylanase 30 A from Clostridium thermocellum functions as a glucuronoxylan xylanohydrolase.
- Sawhney, Neha; Crooks, Casey; Chow, Virginia; Preston, James F.; St. John, Franz. 2016. Genomic and transcriptomic analysis of carbohydrate utilization by Paenibacillus sp. JDR-2: systems for bioprocessing plant polysaccharides.
- Illman, Barbara L.; Sedlmair, Julia; Unger, Miriam; Crooks, Casey; Oliveira, Marli; Hirschmugl, Carol. 2015. Chemistry of wood in 3D: new infrared imaging.
- St John, Franz J.; Dietrich, Diane; Crooks, Casey; Pozharski, Edwin; González, Javier M.; Bales, Elizabeth; Smith, Kennon; Hurlbert, Jason C. 2014. A novel member of glycoside hydrolase family 30 subfamily 8 with altered substrate specificity.
- Sawhney, Neha; Crooks, Casey; St. John, Franz; Preston, James F.; Kelly, R. M. 2014. Transcriptomic Analysis of Xylan Utilization Systems in Paenibacillus sp.
- Dietrich, Diane; Illman, Barbara; Crooks, Casey. 2013. Differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from Burkholderia cepacia and Pseudomonas pseudoflava.
- Ryu, Jae San; Shary, Semarjit; Houtman, Carl J.; Panisko, Ellen A.; Korripally, Premsagar; St. John, Franz J.; Crooks, Casey; Siika-aho, Matti; Magnuson, Jon K.; Hammel, Kenneth E. 2011. Proteomic and functional analysis of the cellulase system expressed by Postia placenta during brown rot of solid wood.
- Fan, Jun; Crooks, Casey; Creissen, Gary; Hill, Lionel; Fairhurst, Shirley; Doerner, Peter; Lamb, Chris. 2011. Pseudomonas sax genes overcome aliphatic isothiocyanate-mediated non-host resistance in Arabidopsis.
|Characterization of Microbial Biocatalysts in Lignocellulosic Utilization|
Bioconversion of lignocellulosic biomass would benefit from development of second generation bacterial biocatalysts. The bacterium Paenibacillus ...
|Development of Enzymes for use in Lignocellulose Processing|
The ultimate best use of lignocellulose allows for the selective extraction of defined value streams. To facilitate this, Forest Service researc ...
|Enzymatic conversion of xylan into valuable products.|
Xylan represents a vast store of potential value but rather than seeing beneficial use, much of this reservoir is lost in catastrophic wildfires ...
|Protein Structure and Biochemical Characterization of a Novel Functioning Xylanase|
Scientists identified and characterized a xylanase with unique function that may have applications in processing of woody biomass substrate.