The Municipality of Santo Domingo, in partnership with Forest Service scientists, the University of Puerto Rico-Río Piedras (UPR), USAID, and the “Instituto Tecnológico de Santo Domingo” (INTEC), is exploring the potential of green infrastructure as a climate adaptation strategy with the use of i-Tree as a tool to quantify green infrastructure distribution and ecosystems services provision. The team of Forest Service and UPR scientists visited Santo Domingo and trained 10 planners and field technicians from the Santo Domingo’s National District Municipality and 15 students and faculty from the INTEC, Ministry of the Environment and conservation NGOs in the use of the i-Tree tool. In the spring of 2016, training participants completed a field survey of 30 plots in the historic Colonial City of Santo Domingo and 20 plots on the INTEC campus. A total of 9762 plants of 76 different species were found in the Colonial City plots. This i-Tree effort was very successful and there are now plans underway to implement 200 plots in the rest of the National District of Santo Domingo. This is the first time the i-Tree tool has been implemented in the Dominican Republic and the effort is serving as a pilot study for i-Tree in other Caribbean and Latin American cities where the use of the tool has been very limited.