Rocky Mountain Research Station Publications

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RM-GTR-297: Micropropagation, genetic engineering, and molecular biology of Populus

Klopfenstein, N. B.; Chun, Y. W.; Kim, M. -S.; Ahuja, M. A., eds. Dillon, M. C.; Carman, R. C.; Eskew, L. G., tech. eds. 1997. Micropropagation, genetic engineering, and molecular biology of Populus. Gen. Tech. Rep. RM-GTR-297. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Forest and Range Experiment Station. 326 p.

Thirty-four Populus biotechnology chapters, written by 85 authors, are comprised in 5 sections: 1) in vitro culture (micropropagation, somatic embryogenesis, protoplasts, somaclonal variation, and germplasm preservation); 2) transformation and foreign gene expression; 3} molecular biology (molecular/genetic characterization); 4) biotic and abiotic resistance (disease, insect, and pollution); and 5) biotechnological applications (wood properties, flowering, phytoremediation, breeding, commercialization, economics, and bioethics).

Keywords: plant transformation, gene expression, tree biotechnology, molecular genetics, bioethics, plant tissue culture

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PDF File Size: 42.6 MB

Download RM-GTR-292 by Chapters

PDF File Size: 180 K

Ned B. Klopfenstein, Young Woo Chun, Mee-Sook Kim, M. Raj Ahuja
PDF File Size: 140 K

Introduction - Sentinels of the Prairie Speak (by Populus Species)
Harold S. McNabb, Jr.
PDF File Size: 720 K

Section I. In Vitro Culture

Chapter 1 - Poplar Shoot Cultures: Their Generation and Use in Biotechnology
Brent H. McCown
PDF File Size: 850 K

Chapter 2 - Somatic Embryogenesis From Poplar Leaf Tissue
Young Goo Park and Sung Ho Son
PDF File Size: 340 K

Chapter 3 - Plant Regeneration Through Organogenesis in Poplar
Hoduck Kang and Young Woo Chun
PDF File Size: 1.4 MB

Chapter 4 - Protoplast Isolation and Culture
Julie Russell Kikkert
PDF File Size: 875 K

Chapter 5 - Somaclonal Variation in Populus: An Evaluation
D. John Fry, Gerry C. Douglas, and N. Th. Saieed
PDF File Size: 1.1 MB

Chapter 6 - Germplasm Preservation of Populus Through In Vitro Culture Systems
Sung Ho Son, Young Goo Park, Young Woo Chun, and Richard B. Hall
PDF File Size: 1.1 MB

Section II. Transformation and Foreign Gene Expression

Chapter 7 - Agrobacterium-mediated Transformation of Populus Species
Mee-Sook Kim, Ned B. Klopfenstein, and Young Woo Chun
PDF File Size: 1.5 MB

Chapter 8 - Direct Gene Transfer in Poplar
Pierre J. Charest, Yvonne Devantier, Cathy Jones, Jim C. Sellmer, Brent H. McCown, and David. D. Ellis
PDF File Size: 610 K

Chapter 9 - Gene Expression Studies
Lise Jouanin and Gilles Pilate
PDF File Size: 470 K

Chapter 10 - Differential Expression of aroA Gene in Transgenic Poplar: Influence of Promoter and Ozone Stress
David F. Karnosky, Gopi K. Podila, Dongill Shin, and Don E. Riemenschneider
PDF File Size: 925 K

Chapter 11 - Growth and Development Alteration in Transgenic Populus: Status and Potential Applications
Bjorn Sundberg, Hannele Tuominen, Ove Nilsson, Thomas Moritz, C. H. Anthony Little, Goran Sandberg, and Olof Olsson
PDF File Size: 2.7 MB

Chapter 12 - Transgene Expression in the Field-Grown Poplar
Gilles Pilate, David D. Ellis, and Simon Hawkins
PDF File Size: 665 K

Chapter 13 - Transgenes and Genetic Instability
M. Raj Ahuja
PDF File Size: 1.9 MB

Section III. Molecular Biology

Chapter 14 - Applications of Molecular Marker Technologies in Populus Breeding
Maria Teresa Cervera, Marc Villar, Patricia Faivre-Rampant, Marie-Christine Goue, Marc Van Montagu, and Wout Boerjan
PDF File Size: 1.7 MB

Chapter 15 - Differentiation of Poplar Clones Using Random Amplified Polymorphic DNA Fingerprinting
Dolly C. Lin, Martin Hubbes, and Louis Zsuffa
PDF File Size: 1.2 MB

Chapter 16 - Seasonal Vegetative Storage Proteins of Poplar
Gary D. Coleman
PDF File Size: 795 K

Chapter 17 - Poplar Seed Storage Proteins
Tannis L. Beardmore, Suzanne Wetzel, and Sharon M. Regan
PDF File Size: 1.5 MB

Chapter 18 - Molecular Genetic Analysis of Populus Chloroplast DNA
Eun W. Noh and Jae S. Lee
PDF File Size: 1.7 MB

Chapter 19 - Molecular Marker Techniques to Study Variability of Populus Pathogens
Girma M. Tabor and Harold S. McNabb, Jr.
PDF File Size: 1.2 MB

Section IV. Biotic and Abiotic Resistance

Chapter 20 - In Vitro Screening and Selection for Disease Resistance
Michael E. Ostry
PDF File Size: 710 K

Chapter 21 - Transformation of Hybrid Aspen for Resistance to Crown Gall Disease
Hiroyasu Ebinuma, Etsuko Matsunaga, Keiko Yamada, and Mikiko Yamakado
PDF File Size: 845 K

Chapter 22 - Designing Small Antimicrobial Peptides and Their Encoding Genes
William A. Powell and Charles A. Maynard
PDF File Size: 2.3 MB

Chapter 23 - Potential of Proteinase Inhibitors for Enhanced Resistance to Populus Arthropod and Pathogen Pests
Scott A. Heuchelin, Lise Jouanin, Ned B. Klopfenstein, and Harold S. McNabb, Jr.
PDF File Size: - K

Chapter 24 - Expression of Transgenic Bacillus thuringiensis delta-endotoxin in Poplar
David D. Ellis and Kenneth F. Raffa
PDF File Size: 1 MB

Chapter 25 - Genetic Engineering for Air-Pollutant Resistance in Hybrid Aspen
Saori Endo, Etsuko Matsunaga, Keiko Yamada, and Hiroyasu Ebinuma
PDF File Size: 890 K

Section V. Biotechnological Applications

Chapter 26 - Genetic Modification of Lignin Biosynthesis in Quaking Aspen and Poplar
Wout Boerjan, Marie Baucher, Brigitte Chabbert, Michel Petit-Conil, Jean-Charles Leple, Gilles Pilate, Daniel Cornu, Bernard Monties, Dirk lnze, Jan Van Doorsselaere, Lise Jouanin, and Marc Van Montagu with Chung-Jui Tsai, Gopi K. Podila, C.P. Joshi, and Vincent L. Chiang
PDF File Size: 1.5 MB

Chapter 27 - Potential Use of Populus for Phytoremediation of Environmental Pollution in Riparian Zones
Mary Ellen Dix, Ned B. Klopfenstein, Jian-Wei Zhang, Sarah W. Workman, and Mee-Sook Kim
PDF File Size: 655 K

Chapter 28 - Poplar Genetically Engineered for Reproductive Sterility and Accelerated Flowering
Richard Meilan and Steven H. Strauss
PDF File Size: 815 K

Chapter 29 - Integration of Molecular and Classical Genetics: A Synergistic Approach to Tree Improvement
Ronald J. Dinus and Gerald A. Tuskan
PDF File Size: 1.6 MB

Chapter 30 - Application of Tissue Culture Systems for Commercial Plant Production
Kathryn A. Louis and LoriAnn E. Eils
PDF File Size: 1.3 MB

Chapter 31 - Economics of Producing Populus Biomass for Energy and Fiber Systems
Charles H. Strauss and Stephen C. Grado
PDF File Size: 680 K

Chapter 32 - Environmental Risk Assessment and Deployment Strategies for Genetically Engineered Insect-Resistant Populus
Kenneth F. Raffa, Karl W. Kleiner, David D. Ellis, and Brent H. McCown
PDF File Size: 1.5 MB

Chapter 33 - Biotechnology Risk Assessment: A View From Plant Pathology
X. B. Yang
PDF File Size: 940 K

Chapter 34 - Biotechnology, Biodiversity, and Bioethics
M. Raj Ahuja
PDF File Size: 1.1 MB

Section VI. Appendices

Authors and Affiliations
PDF File Size: 400 K

Reviewer Acknowledgment
PDF File Size: 120 K

Scientific and Common Names of Biological Organisms Associated With Populus Biotechnology
Madelyn C. Dillon, Richard C. Carman, and Mee-Sook Kim
PDF File Size: 420 K

Glossary of Populus Tissue Culture and Molecular Biology
Mee-Sook Kim, Madelyn C. Dillon, and Richard C. Carman
PDF File Size: 1.5 MB

Index of Populus
PDF File Size: 165 K

General Index
PDF File Size: 1.3 MB

Title: RM-GTR-297: Micropropagation, genetic engineering, and molecular biology of Populus
Electronic Publish Date: October 19, 2012
Last Update:
October 19, 2012

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