Rocky Mountain Research Station Publications

RMRS Online Publication - Journal Articles, External Publications, and Special Reports
Virulence of Fusarium oxysporum and F. commune to Douglas-fir (Pseudotsuga menzeisii) seedlings


Stewart, J. E.; Abdo, Z.; Dumroese, R. K.; Klopfenstein, N. B.; Kim, M. -S. 2012. Virulence of Fusarium oxysporum and F. commune to Douglas-fir (Pseudotsuga menzeisii) seedlings. Forest Pathology. 42: 220-228.

doi: 10.1111/j.1439-0329.2011.00746.x

Fusarium species can cause damping-off and root rot of young conifer seedlings, resulting in severe crop and economic losses in forest nurseries. Disease control within tree nurseries is difficult because of the inability to characterize and quantify Fusarium spp. populations with regard to disease potential because of high variability in isolate virulence. Fusarium isolates were collected from healthy and diseased seedlings of Douglas-fir (Pseudotsuga menziesii) and western white pine (Pinus monticola) from a nursery in Idaho, USA. Molecular markers such as DNA sequences (mitochondrial small subunit and nuclear translation elongation factor 1-alpha) and amplified fragment length polymorphism were used to identify isolates as either F. oxysporum or F. commune. In addition, diagnostic primers were developed to detect and distinguish F. commune from F. oxysporum. In vitro and greenhouse virulence tests were completed on Douglas-fir germinants and seedlings. For Douglasfir germinants and seedlings, F. oxysporum isolates generally caused less severe symptoms, whereas most F. commune isolates caused mortality through damping-off. This is the first report of direct evidence that F. commune can cause damping-off disease on Douglas-fir seedlings under greenhouse conditions.

Keywords: Fusarium, Douglas-fir, Pseudotsuga menzeisii, seedlings


About PDFs: For best results, do not open the PDF in your Web browser. Right-click on the PDF link to download the PDF file directly to your computer. Click here for more PDF help.


Download Article
http://www.fs.fed.us/rm/pubs_other/rmrs_2012_stewart_j001.pdf

PDF File Size: 725 K


Title: RMRS Other Publications: Virulence of Fusarium oxysporum and F. commune to Douglas-fir (Pseudotsuga menzeisii) seedlings
Electronic Publish Date: August 22, 2012
Last Update:
August 22, 2012

RMRS Publications | Order a publication | Contact Us