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Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets


Brinkman, Todd J.; Person, David K.; Schwartz, Michael K.; Pilgrim, Kristine L.; Colson, Kevin E.; Hundertmark, Kris J. 2010. Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets. Conservation Genetics Resources. 2: 115-118.

We tested a protocol for extracting DNA from fecal pellets from Sitka black-tailed deer (Odocoileus hemionus sitkensis) and evaluated genotyping performance of previously developed microsatellite markers as well as a suite of new markers designed specifically for this study. We screened 30 microsatellites, and identified 7 (23%) loci including 4 new markers, that fit well into a single multiplex and consistently genotyped deer with low error rates. DNA was extracted from 2,408 fecal-pellet samples. Of those, 1,240 (52%) were genotyped successfully at all 7 markers allowing identification of 634 genetically unique deer. Using DNA from fecal pellets collected in the field was an effective technique for identifying and distinguishing among deer.

Keywords: Alaska, DNA, feces, microsatellites, Odocoileus hemionus sitkensis, Sitka black-tailed deer


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http://www.fs.fed.us/rm/pubs_other/rmrs_2010_brinkman_t001.pdf

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Title: RMRS Other Publications: Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets
Electronic Publish Date: March 15, 2012
Last Update:
March 15, 2012

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