Rocky Mountain Research Station Publications

RMRS Online Publication - Journal Articles, External Publications, and Special Reports
Ribosomal DNA, heterochromatin, and correlation with genome size in diploid and polyploid North American endemic sagebrushes (Artemisia, Asteraceae)


Garcia, Sonia; Garnatje, Teresa; Pellicer, Jaume; McArthur, E. Durant; Siljak-Yakovlev, Sonja; Valles, Joan. 2009. Ribosomal DNA, heterochromatin, and correlation with genome size in diploid and polyploid North American endemic sagebrushes (Artemisia, Asteraceae). Genome. 52: 1012-1024.

Subgenus Tridentatae (Artemisia, Asteraceae) can be considered a polyploid complex. Both polyploidy and hybridization have been documented in the Tridentatae. Fluorescent in situ hybridization (FISH) and fluorochrome banding were used to detect and analyze ribosomal DNA changes linked to polyploidization in this group by studying four diploidpolyploid species pairs. In addition, genome sizes and heterochromatin patterns were compared between these populations. The linked 5S and 35S rRNA genes are confirmed as characteristic for Artemisia, and a pattern at the diploid level of three rDNA loci located at telomeric positions proved to be typical. Loss of rDNA loci was observed in some polyploids, whereas others showed additivity with respect to their diploid relatives. Genome downsizing was observed in all polyploids. Banding patterns differed depending on the pair of species analysed, but some polyploid populations showed an increased number of heterochromatic bands. FISH and fluorochrome banding were useful in determining the systematic position of Artemisia bigelovii, for which a differential pattern was found as compared with the rest of the group. Additionally, FISH was used to detect the presence of the Arabidopsis-type telomere repeat for the first time in Artemisia.

Keywords: allopolyploidy, Arabidopsis-type telomere, autopolyploidy, Compositae, chromomycin, DAPI, FISH, fluorochrome banding, genome organization, Tridentatae


About PDFs: For best results, do not open the PDF in your Web browser. Right-click on the PDF link to download the PDF file directly to your computer. Click here for more PDF help.


Download Article
http://www.fs.fed.us/rm/pubs_other/rmrs_2009_garcia_s001.pdf

PDF File Size: 295 K


Title: RMRS Other Publications: Ribosomal DNA, heterochromatin, and correlation with genome size in diploid and polyploid North American endemic sagebrushes (Artemisia, Asteraceae)
Electronic Publish Date: November 30, 2010
Last Update:
November 30, 2010

RMRS Publications | Order a publication | Contact Us