Rocky Mountain Research Station Publications

RMRS Online Publication - Journal Articles, External Publications, and Special Reports
Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)


Davis, C. S.; Mock, K. E.; Bentz, B. J.; Bromilow, S. M.; Bartell, N. V.; Murray, B. W.; Roe, A. D.; Cooke, J. E. K. 2009. Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae). Molecular Ecology Resources. doi: 10.1111/j.1755-0998.2009.02579.x

We isolated 16 polymorphic microsatellite loci in the mountain pine beetle (Dendroctonus ponderosae Hopkins) and developed conditions for amplifying these markers in four multiplex reactions. Three to 14 alleles were detected per locus across two sampled populations. Observed and expected heterozygosities ranged from 0.000 to 0.902 and from 0.100 to 0.830, respectively. Three loci deviated from Hardy-Weinberg equilibrium in one sampled population. One of these loci may be sex linked. These markers will be useful in the study of population structure in this important pest species.

Keywords: Dendroctonus ponderosae, microsatellite, mountain pine beetle


About PDFs: For best results, do not open the PDF in your Web browser. Right-click on the PDF link to download the PDF file directly to your computer. Click here for more PDF help.


Download Article
http://www.fs.fed.us/rm/pubs_other/rmrs_2009_davis_c001.pdf

PDF File Size: 80 K


Title: RMRS Other Publications: Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)
Electronic Publish Date: November 16, 2009
Last Update:
November 16, 2009

RMRS Publications | Order a publication | Contact Us