Dorena Genetic Resource Center

Rustbusters2003 - Symposium on Blister Rust Resistance

Agenda (subject to change)

As of 7/16/2003

Fieldtrips

All sites will be on Cottage Grove Ranger District on the Umpqua National Forest. Several sites will have infected Ribes and the ‘Champion Mine Strain’ will be predominant.

Weather can vary (usually warm to hot; a remote chance of rain). One of the sites (Grass Creek) will require some uphill hiking.

Saturday (Aug. 16) Fieldtrip #1: 0900-1600

BRING SACK LUNCH

  1. Visit to Crawfish/Dog Creek site—see large natural sugar pine and western white pine, pine regeneration, and blister rust infection.
  2. Grass Creek Area (Bob Danchok, Richard Sniezko, Bro Kinloch)
    • 1969-1974 full-sib plantings
    • 30+ years of rust infection
  3. Champion Mine Area—1956 to 2003 (Jerry Barnes, Bro Kinloch and Bob Danchok)
    • Champion Mine: the power of natural selection
    • The first wave: Cr2 revealed
    • The second wave: vcr2 ascendant
    • Aftermath
  4. East Fork 1998 Pinus monticola rust resistance field test (heavy infection) - may be Saturday or Sundat (S. Kolpak, B. Danchok, R. Sniezko).
  5. Ribes Garden Visit (may be on Sat. or Sunday)

Sunday (Aug. 17) Fieldtrip #2: 0900-1600

BRING SACK LUNCH

Morning—Field
  1. Visit Ker-Bluey 1996 validation test (12 SP, 13 WWP families)—heavy infection (R. Sniezko/B. Danchok/S. Kolpak)
  2. Visit other tests (time permitting)
Afternoon—Dorena
  1. Examine rust screening trials (WWP, SP, EWP, WBP, and other species). (R. Sniezko/A. Kegley/R. Danchok)
  2. Dorena Inoculation Chamber (B. Danchok/S. Long/R. Spence)
  3. Dorena Orchards and Breeding efforts (R. Sniezko/J. Danielson/S. Long)
  4. MGR testing at Dorena (S. Long/R. Danchok/R. Sniezko)
  5. Breeding for Phytopthora lateralis resistance in Port-Orford-cedar (R. Sniezko/L. Elliot/S. Kolpak)

Monday (Aug. 18th)—INDOOR DISCUSSION SESSION

Approximately 0800 to 1600. (Meeting will end at 1600 to allow some folks chance to drive to Grants Pass for WIFDWC)

Will be held at Cottage Grove Ranger Station
  1. Round table updates of projects/programs.
  2. Discussion Topics (see draft listing below)—we will keep formal presentation to a minimun
  1. Blister Rust Resistance Mechanisms/Responses- Current knowledge and information Needed (Discussion Facilitators: Richard Sniezko/ Bro Kinloch/Paul Zambino, others)
    1. Major Gene Resistance
      1. (Cr1, Cr2, Cr3)
      2. Virulence to major gene resistance
      3. other? (Mechanism 'X')
    2. Partial Resistance (inheritance uncertain)
      1. Previous Standard putative mechanisms
      2. 'Q' and 'Q2'
    3. Eastern white pine (P. Zambino, R. Sniezko)
    4. Virulence/resistance and 'Fitness' of pathogen and host (Dorena examples?)
    5. Other
    6. Terminology Issues
  2. Field Results and Durability of Resistance (Bro Kinloch, Richard Sniezko, and John Schwandt, Tom Kubisiak)
    • Inter-regional test at Happy Camp (The white pine inter-regional test: outcome of three decades of genetic interactions among host resistance and pathogen virulence genes)
    • R6 plantings
    • R1/RMRS plantings?
    • Canadian plantings?
    • Field Results vs. short-term screening results for WWP & SP
  3. Inoculation Issues (R.Sniezko, A.Kegley, and others)
    1. traditional operational testing
      • repeatability of results
      • inoculum levels
      • source of inoculum
      • modifications for the future?
    2. MGR testing
    3. new testing methods?
    4. 'new' species
  4. Ribes issues (commercial cultivation of Ribes in North America, Ribes resistance to C. ribicola) (Paul Zambino and others)
    • resistance in commercial and wild Ribes in North America
    • implications of commercial Ribes cultivation and Ribes regrowth after management practices
    • possibility that virulence on a host affects aggressiveness on that or other hosts through balancing selection, potential effects of Ribes proliferation after fire or management.
    • testing at Dorena
  5. Genetic Variation in Cronartium ribicola (molecular and other) - ramnifications for resistance breeding in pines (also see #6 below)
    • Genetic Variation in C. ribicola affecting different white pine species
    • to effectively manage these types of pathosystems we need to be able to monitor avr frequencies in natural populations of the pathogen so as to make more informed decisions on host R-gene deployment
    • strategies for mapping avr genes in gene-for-gene systems
    • creating segregating lines of the pathogen
    • Genetic Variation in Cronartium ribicola, proposing effects of introduction history in Europe and North America
    • Implications for stabilization of pathosystems and utilization of selected vs. natural resistance in pines
  6. Molecular Tools (Tom Kubisiak, Bryce Richardson, Mee-Sook Kim, Paul Zambino, Abul Ekramoddoullah, others)
    • Uses of molecular markers
      • Use of genetic markers to assess genetic diversity of western white pine and Cronartium ribicola
      • Use of genetic markers to assess changes in the blister rust pathosystem over time, and evaluate potential for natural recovery of western white pine
      • Use of molecular markers for resistance responses or mechanisms in breeding programs (e.g. slow-canker growth/bark reaction)
      • use of molecular markers (specifically SSRs in C.q.f.) for fungal line fingerprinting/identification and verification of fungal crosses
      • use of molecular markers to study infection under natural versus artificial inoculation conditions
    • Gene-for-gene systems
      • the need for studying both host and pathogen in gene-for-gene systems
      • use of molecular markers in both host and pathogen to better understand the genetics of the gene-for-gene interaction (touch on use of various marker types such as RAPD's, AFLP's, and SSR's)
      • a topic for discussion - should we even be worried about mapping R-genes and avr genes and just plant mixed families of resistant families (similar approach to what has been done for coffee rust in SA)
      • microarrays to identify differentially expressed genes in both the host and pathogen that may likely be involved in compatible and incompatible interactions.
      • Isolation and characterization of R genes and their potential use in breeding (e.g. Cr1, Cr2 etc) and also the possibility of 'stacking' these genes together in white pines.
      • Search for R-genes and avr genes in crosses of hosts and pathogens.
  7. Non-North American host species and Hybrids (John King, R. Sniezko)
    • Resistance
    • Potential
  8. ‘Wild Theories’
    1. Lack of MGR in EWP (R. Sniezko)
    2. Uphill battle in North America (R. Sniezko)
  9. Strategies for the future development of resistant populations (Sniezko and others)
    • WWP and SP
    • EWP
    • Other species (whitebark pine, limber pine, foxtail pine, southwestern white pine, both bristlecone pine species)
    • But we need these trees now
      • 'Instant restoration' - field selection, cone collection and deployment of untested material. Will it work?
  10. Current and Needed Links between Research and Development of resistant populations
    • materials needed
  11. Funding options
  12. Needs
  13. (NEW) National Report on white pines and C. ribicola (WO represantatives).
  14. Summary
Dorena Genetic Resource Center
34963 Shoreview Road
Cottage Grove, OR 97424
Phone: (541) 767-5700
Fax: (541) 767-5709

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