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Research Note
Title: Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis
Author: Conkle, M. Thompson
Date: 1972
Source: Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest and Range Experiment Station. 6 p
Station ID: RN-PSW-264
Description: Enzymes in forest tree materials can be resolved by starch gel electrophoresis. A gel slab is prepared in a mold assembled from glass and plastic. Wicks containing an aqueous extract of macerated plant material are inserted in the gel and processed. The gel is sliced, stained, examined, and photographed. Isozyme bands produced by differential migration of enzymes indicate genetic segregation and recombination. This technique is familiar in other uses, but the procedure described here is specifically adapted to conifers.
Keywords: coniferae; natural variation; genetic diversity; chemical composition; enzyme
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Citation
Conkle, M. Thompson 1972. Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis. Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest and Range Experiment Station. 6 p.