USDA Forest Service
 

Pacific Southwest Research Station

 

Pacific Southwest Research Station
800 Buchanan Street
Albany, CA 94710-0011

(510) 559-6300

United States Department of Agriculture Forest Service. USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.

Publications and Products

Order a printed copy
of this publication.

General Technical Report

Title: Starch gel electrophoresis of conifer seeds: a laboratory manual

Author: Conkle, M. Thompson; Hodgskiss, Paul D.; Nunnally, Lucy B.; Hunter, Serena C.

Date: 1982

Source: Gen. Tech. Rep. PSW-GTR-64. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Forest and Range Experiment Station: 18 p

Station ID: GTR-PSW-064

Description: This manual describes fast, low-cost biochemical procedures for separating enzymes representing numerous genes of forest trees. During electrophoresis the mixture of enzymes from a megagametophyte or embryo of a germinated seed separates in a gel. Specific stains applied to gel slices locate each enzyme. These procedures expand on those developed for crops research. They provide a means for forest geneticists to get urgently needed information on the amount and geographic distribution of genetic variation in conifers for evaluating species relationships, for protecting rare natural populations, and for deciding on breeding programs for commercial species. Electrophoresis of conifers is an alternative to long-term, high-cost field trials.

Key Words: technique, gametophytes, embryos, allozymes, eiectrophoresis

View and Print this Publication (3.7 MB)

Citation

Conkle, M. Thompson; Hodgskiss, Paul D.; Nunnally, Lucy B.; Hunter, Serena C.  1982.  Starch gel electrophoresis of conifer seeds: a laboratory manual  Gen. Tech. Rep. PSW-GTR-64. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Forest and Range Experiment Station: 18 p.

Last Modified: Nov 18, 2013 12:41:53 PM